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Title: Comparison of vaginal microbiota sampling techniques: cytobrush versus swab
Authors: British Society for Colposcopy and Cervical Pathology;Imperial College Healthcare Charity;Genesis Research Trust;Imperial College Healthcare Charity;Mitra, A;Mahajan, V;Macintyre, D;Lee, Y;Smith, A;Marchesi, J;Lyons, D;Bennett, P;Kyrgiou, M
Year: 7-Sep-2017
Publisher: Nature Publishing Group
place: Medical Research Council (MRC);Medical Research Council (MRC)
Description: Evidence suggests the vaginal microbiota (VM) may influence risk of persistent Human Papillomavirus (HPV) infection and cervical carcinogenesis. Established cytology biobanks, typically collected with a cytobrush, constitute a unique resource to study such associations longitudinally. It is plausible that compared to rayon swabs; the most commonly used sampling devices, cytobrushes may disrupt biofilms leading to variation in VM composition. Cervico-vaginal samples were collected with cytobrush and rayon swabs from 30 women with high-grade cervical precancer. Quantitative PCR was used to compare bacterial load and Illumina MiSeq sequencing of the V1-V3 regions of the 16S rRNA gene used to compare VM composition. Cytobrushes collected a higher total bacterial load. Relative abundance of bacterial species was highly comparable between sampling devices (R2 = 0.993). However, in women with a Lactobacillus-depleted, high-diversity VM, significantly less correlation in relative species abundance was observed between devices when compared to those with a Lactobacillus species-dominant VM (p = 0.0049). Cytobrush and swab sampling provide a comparable VM composition. In a small proportion of cases the cytobrush was able to detect underlying high-diversity community structure, not realized with swab sampling. This study highlights the need to consider sampling devices as potential confounders when comparing multiple studies and datasets.
Standard no: 2045-2322
Type Of Material: Article
Appears in Collections:Department of Surgery and Cancer

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